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June 2020 Vol. 8 No.6

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Izebe KS
Olurinola PF

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Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 8(5) pp. 226-236, May, 2020 

Copyright © 2020 Merit Research Journals
DOI: 10.5281/zenodo.3908619


Original Research Article

Formulated Sorghum Media for Cultivation of Escherichia coli (NCTC10418) and Pseudomonas aeruginosa (NCTC 6750)

 
 
 

Izebe KS1*, Ya’aba Y1, Onaolapo JA2, Ibrahim K1, Ibrahim YKE2, Oladosu P1, Njoku M1, Mohammed SB1,
Ezeunala M1 and Olurinola PF2

 

1Department of Microbiology and Biotechnology, National Institute for Pharmaceutical Research and Development, Idu, Abuja, Nigeria
2Department of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria.

*Corresponding Author's E-mail: alkysim@yahoo.com
Mob.: +234 8054492351

Received: 02 June 2020  I  Accepted: 23 June 2020  I  Published: 28 June 2020  I  Article ID: MRJMMS-20-085
Copyright © 2020 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0.

 

Abstract

 

The study was aimed to formulate sorghum based media for the cultivation of E.coli and P.aeruginosa. The sorghum grains were treated of debris, socked in distilled water and ground into paste. The fibers removed and centrifuged at 9000 rpm for 15 minutes. The protein-like and starch deposited were dried. The glucan and starch were digested with pepsin and amylase enzymes to protein and sugar digest followed by combinations and the pH adjusted to 7.2 and sterilized to obtained sorghum media. sorghum and Nutrient broth were inoculated with diluted 24 hours. of E.coli and P.aeruginosa. The growth of these bacteria were visualized, absorbance (OD) and viable count estimated. A growth profile of E.coli and P.aeruginosa in Nutrient and sorghum media were monitored using spectrophotometric for six hours. The OD0.8 for E. coli and P. aeruginosa in Sorghum media while OD 1.0 and 0.9 for E. coli and P. aeruginosa in Nutrient Broth. The mean viable count of E.coli was 5.6 x 1022±0.01 and 4.9 x 1022±0.004 CFU/mLin Nutrient and sorghum media. While P. aeruginosa mean viable count was 5.1 x 1022±0.011 and 4.0 x1022±0.005 CFU/mL in Nutrient and sorghum media respectively with no growth. The growth curve pattern of E. coli and P. aeruginosa showed no lag phase and lengthen log phase. The findings of this study showed that developed Sorghum media can promote the growth of bacteria in the same way as the Nutrient broth.

Keywords: Absorbance, E.coli, Enzymatic digestion, Growth curve, P.aeruginosa. Sorghum grains, Sorghum media, Turbidity, Viable count, Nutrient broth































 







 








 





















 









































































 










 







































 










 

 
 
   
   
   
   
   
   
   
   
   
   
   
 
 
 
 
 
 
 
 
   
 
                         

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