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June 2020 Vol. 8 No.6

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Akhtar N
Jamil A

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Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 8(6) pp. 237-243, June, 2020 

Copyright © 2020 Merit Research Journals
DOI: 10.5281/zenodo.3908606

Original Research Article

Assessment of Standard Nursing Practices Implementation during Blood Culture Sampling In Emergency Department


Naseem Akhtar1, Muhammad Hussain2, Muhammad Afzal3, Prof. Dr. Syed Amir Gilani4 and Awais Jamil5


1Student of Post RN Year 2, Semester II, (Lahore, Pakistan)
2Nursing Assistant Professor (Lahore, Pakistan)
3Nursing Principal (Lahore, Pakistan)
4DEAN of Allied Health sciences (Lahore, Pakistan)
5Clinical nurse manager (Lahore, Pakistan)

*Corresponding Authorís E-mail: naseem.akhtar.2538@gmail.com

Received: 22 May 2020  I  Accepted: 22 June 2020  I  Published: 28 June 2020
Copyright © 2020 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0.




Blood culture sample is the main resource to identify the causing agent of severe infection and septicemia. It is also used for the appropriation of antibiotic regime. Unfortunately, blood culture contamination produces misperception regarding the importance of a positive result of blood culture. The knowledge of existing guidelines, education session and competency of blood culture sampling are very beneficial to reducing blood culture contamination. The study aimed to enhance nursing knowledge and practices after the blood culture sampling session and a quasi-experimental study design by an educational intervention. The sampling techniques were purposive; the sample size was 50 nurses, working in the emergency department. Its duration was three months. The rigor of study instruments were checked by content validity index 0.91 and reliability by Cronbach alpha 0.9. The study was analyzed through Statistical Package for the Social Sciences (SPSS). The demographic variable, knowledge and practices will be depicted in the form of frequencies and percentages. The score of an educational intervention was assessed by paired t-test. The results showed that there was an association of improved knowledge with educational intervention as the t-score was 11.79 and practices was 9.39. The p-value is significant at 0.000. Sufficient knowledge makes the nurses to be able to perform practices according to set standards and increased their responsibility to be aware of the criticality of every step taken to perform a procedure and their importance. Good practices can reduce the blood culture contamination rate and not to eliminate it.

Keywords: Blood, Practice, Contamination, Knowledge, Intervention, Education










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