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August 2020 Vol. 8 No.8

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Yousif WT
Zuhdi A

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Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 8(8) pp. 403-410, August, 2020 

Copyright © 2020 Author(s) retain the copyright of this article
DOI: 10.5281/zenodo.3996694

Original Research Article

Molecular and biochemical studies on the effect of the infection by cutaneous leishmaniasis in Iraqi people


Walaa Taha Yousif1, Prof Dr. Amany Mohamed Al-kaysi1, Prof Dr. Mohammad Oda Selman2, Dr. Khadija Khaleel Aljuboori2, Dr. Ahmed Zuhdi2


1College of Medical and Health Technology
2Ministry of Health. IRAQ / BAGHDAD

*Corresponding Author's Email: drmohammadoda@gmail.com

Received: 04 August 2020  I  Accepted: 18 August 2020  I  Published: 24 August 2020  I  Article ID: MRJMMS-20-126
Copyright © 2020 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0.




The present study is designed for the detection of cutaneous Leishmania by microscopic and culture methods, also for the characterization of species that are endemic in Iraq using molecular techniques, including conventional and nested polymerase chain reaction. Some biochemical tests including liver and kidney functions tests were also carried out to estimate the levels of Aspartate aminotransferase, Alanine aminotransferase, uric acid and blood urea in serum blood samples of patients before and after administered treatment. The results showed that a high percentage of patients had multiple lesions. One hundred and fifty patients enrolled in this study divided as follows; ninety patients not received any treatment, thirty patients were taken treatments and the other thirty people chose as control group. Using nested polymerase chain reaction techniques showed that the causative agents of cutaneous leishmaniasis in Iraq were two species: mainly L. major and L.tropica. No elevation in the level of ALT (GPT) in serum blood samples were noticed in patients before and after administering treatment. The present study also revealed no elevation in blood urea and uric acid in both groups of patients before and after administering treatment. Slight increases were noted in the values of Aspartate aminotransferase, Alanine aminotransferase in serum blood samples of patients before administered treatment compared with healthy control group, but there were no elevations in the values of Alanine aminotransferase in serum blood samples in both groups.

Keywords: Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Leishmania, Polymerase chain reaction











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