November 2019 Vol. 7 No.11

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Original Research Article

Phytochemical investigation and Antimicrobial activity of Sudanese Faidherbia albida and Khaya senegalensis bark extracts and their combination against wound infection pathogens

Mojahid Mohammed Khalid¹, Einas Gamal Mohieldin², Afraa Mubarak Alhaj³ and Ayat Ahmed Alrasheid^1*

¹Pharmacognosy Department, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan.
²Pharmacology Department, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan.
³Microbiology Department, Faculty of Medical Laboratory Sciences, University of Medical Sciences and Technology, Khartoum, Sudan.

*Corresponding Author's E-mail: ayatwarag@yahoo.com

Accepted November 13, 2019

Abstract

Antimicrobial resistance has become an important public health problem in Sudan. Some bacteria resistant to developed antibiotics. This study was conducted on the ethanolic bark extracts of Sudanese Faidherbia albida and Khaya senegalensis and their combination to carry out phytochemical screening and to evaluate the antimicrobial activity with the determination of the minimum inhibitory concentration. Qualitative phytochemical screening of ethanolic extract of the bark of F. albida indicated the presence of sterols, tannins and alkaloids, while the ethanolic extract of the bark of K. senegalensis contain alkaloids, triterpenes, tannins, saponins, cardiac glycosides, and reducing sugars. The total tannins content of the ethanolic bark extracts was determined using double beam Spectrophotometer. The result was found to be 9.099 ppm for F. albida, 9.63 ppm for K. Senegalensis and 10.983 ppm for the combination of both extracts (1:1). Antimicrobial activity and minimum inhibitory concentration for the both samples was determined using disc diffusion method. It was found that there is higher activity against the two Gram positive bacteria and Proteus spp Gram negative bacteria. than Klebsiella spp. Generally K. senegalensis showed slightly higher antibacterial activity than F.albida, but it showed low antifungal activity. Further researches are needed for the identification and isolation of the compounds responsible for the antimicrobial activity.

Keyword: F. albida, K. senegalensis, wound, antimicrobial activity, tannin