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December 2018 Vol. 6 No.12

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Triantafyllopoulou D
Kyrtsonis M

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Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 6(12) pp. 432-439, December, 2018 

Copyright © 2018 Merit Research Journals

Original Research Article

Bone marrow osteoprotegerin (OPG) and interleukin-8 (IL-8) immunostaining in patients with Ph-negative myeloproliferative neoplasms (MPN): Clinical correlations


Diana Triantafyllopoulou1*, Dimitrios Maltezas1*, Eleni-Andrianna Trigka2, Tatiana Tzenou1, Theodoros Iliakis1, Maria Dimou1, Aspasia Koudouna1, Kallirroi Spanou2, Panagiota Ntailiani2, Efstratios Patsouris2, Maria K. Angelopoulou3, Maria Kotsopoulou, Panayiotis Panayiotidis1, Penelope Korkolopoulou2* and Marie-Christine Kyrtsonis1*


1Haematology Section of First Department of Propaedeutic Internal Medicine, Laikon Hospital, National and Kapodistrian University of Athens’ Medical School, Athens, Greece
2Department of Pathology, National and Kapodistrian University of Athens’ Medical School, Athens, Greece
3Department of Haematology, National and Kapodistrian University of Athens’ Medical School, Athens, Greece

*Corresponding Author Email: mck@ath.forthnet.gr

Accepted December 17, 2018




We evaluated the expression and intensity of IL-8 and OPG immunostaining in BM biopsies of MPN patients at diagnosis and correlated the results with clinical parameters. Seventy-six patients were studied, diagnosed with MPN from 1984 to 2012 with a median follow up period of 55 months. IL-8 and OPG immunostaining was performed on paraffin-embedded sections of formalin fixed BM biopsies, carried out at the time of diagnosis, using the two-step peroxidase conjugated polymer technique. Grade of positivity and intensity of IL-8 and OPG expression was scored according to a 0 to 3 scale. Statistical analysis was performed conventionally, using the SPSS version 22.0 package, and p values <0.05 were considered significant. High intensity of IL-8 expression in megakaryocytes correlated, in the whole cohort, with neutrophilia (p=0.005) and high haemoglobin (p=0.001). High intensity of OPG expression in megakaryocytes correlated high haemoglobin (p=0.017) and a favorable karyotype (p=0.01). Most importantly, low OPG intensity in megakaryocytes correlated with poor survival (p=0.026) in the whole patient series. In conclusion, our most important finding is that high intensity of OPG expression in megakaryocytes correlated with increased survival and a favorable karyotype in patients with MPN, a finding that has not been reported yet.

Keywords: Myeloproliferative Neoplasms (MPN), Polycythemia Vera (PV), Essential Thrombocytosis (ET), Primary Myelofibrosis (MF), Osteoprotegerin (OPG), Interleukin-8 (IL-8)






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