
March
2021 Vol.9 No.1
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Merit Research Journal of Microbiology and Biological Sciences
(ISSN: 2408-7076) Vol.
9(1) pp. 001-008, March, 2021
Copyright © 2021 Author(s) retain the copyright
of this article
DOI: 10.5281/zenodo.4661556 |
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Original Research Article
Association between virulence factors and
antibiotic resistance in E.coli isolated from urinary
tract infection patients in Banha University Hospitals, Egypt |
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1Microbiology
and Immunology Department, Faculty of Medicine, Benha
University, Egypt
2Urology Department, Faculty of Medicine,
BenhaUniversity,Egypt
*Corresponding Author's Email: drenaskhater@yahoo.com
Received: 28 February 2021 I Accepted:
26 March 2021 I Published:
29 March 2021
I Article ID: MRJMBS21012
Copyright © 2021 Author(s) retain the
copyright of this article.
This article is published under the terms of the
Creative Commons Attribution
License 4.0. |
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This study aimed to assess the antibiotic resistance pattern
among E. coli isolated from patients with UTI, to detect
the presence of virulence factors and biofilm formation and to
evaluate the relation between virulence factors and antibiotic
resistance. A cross sectional study was performed in Banha
University Hospitals, Egypt from May to November, 2020. Urine
samples were obtained from included patients. Vitek-2 was used
for identification of isolates and performing antimicrobial
susceptibility testing. Congo red agar method was used to detect
biofilm formation. Isolated Escherichia coli strains were
screened for harboring virulence genes; Pap C, Fim H, Sfa,
Afa, Hly A, and Cnf1 using multiplex PCR. E.coli
isolates showed highest resistance against ampicillin 86(96.6%)
followed cefazolin 85(95.5%), while the most susceptibility was
to both imipenem and meropenem 85(95.6%). Sixty two (67.7%)
isolates were multidrug-resistant. Biofilm formation was
detected in 54(60.7%) isolates. FimH has the highest
prevalence 63(70.8%) among E. coli isolates, followed by
Pap C 37(41.6%) and the least was Cnf 1 genes
2(2.2%). The prevalence of the FimH gene was markedly
higher in the strains resistant to ampicillin, cefazolin,
ceftazidime and ceftriaxone in comparison to the susceptible
strains. The Pap C gene was related to resistance to
ceftazidime, amoxicillin/clavulanic acid and Piperacillin/
Tazobactam. The Cnf1 gene was related to resistance to
amoxicillin/ clavulanic acid and nitrofurantoin. There was a
statistically significant difference between biofilm production
and resistance to ampicillin, ceftriaxone, ceftazidime and
ciprofloxacin. In conclusion multi drug resistant E. coli
showed high prevelance among isolates 62(67.7%) which is a
significant clinical challenge. The highest prevalence of
virulence genes among the E coli strains such as FimH and
PapC, suggesting their importance in pathogenesis of UTI
caused by E coli. Relation between the virulence factors
and antimicrobial resistance should be periodically evaluated in
each health care facilities.
Keywords: Biofilm, Escherichia coli, UTI,
Virulence factors
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