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MERIT RESEARCH JOURNAL OF  AGRICULTURAL SCIENCE AND SOIL SCIENCE (MRJASSS) (ISSN: 2350-2274)

 
 

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March 2021 Vol. 9 No.3

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Mohamed NT
Essam W

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Merit Research Journal of Agricultural Science and Soil Science (ISSN: 2350-2274) Vol. 9(3) pp. 016-028, March, 2021

Copyright © 2021 Author(s) retain the copyright of this article
DOI:
10.5281/zenodo.4644516

Original Research Article

Characterization of serine proteases and glycosidases using enzyme assay in the mid-gut of adult Trachyderma philistine (Coleoptera: Tenebrionidae)

 
 
 

Nancy Taha Mohamed1*, Ahmed Salem El-Ebiarie2, Waffa Essam3

 

1Zoology and Entomology Department, Faculty of Science, Helwan University.
2Zoology & Entomology Department, Faculty of Science, Helwan University.
3Zoology & Entomology Department, Faculty of Science, Helwan University.

*Corresponding Author Email: nancyt0000@yahoo.com
Telephone: (002) 0100 546 0968
 

Received: 11 December 2020 
I  Accepted: 26 January 2021  I  Published: 29 March 2021  I  Article ID: MRJASSS20051
Copyright © 2021 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0.

 

Abstract

 

The biochemical activities of some digestive enzymes produced by adult Trachyderma philistine beetle were studied. The mid-gut of adult stage of Trachyderma philistine was separated into anterior, middle, and posterior parts. The total protein of anterior mid-gut was 3.34 mg/dl, and that of middle mid-gut was 2.53 mg/dl and the total protein of posterior mid-gut was 3.21 mg/dl. The highest activity of trypsin was at pH 8 in anterior mid-gut, middle mid-gut, and posterior mid-gut. The highest activity of chymotrypsin in anterior mid-gut was at pH 6, while at pH 7 in middle and posterior mid-guts. Leucine – amino peptidase showed activity at pH 8 in 3 portions of midguts. The carboxypeptidase A showed activity at pH 8 in both the anterior mid-gut and middle mid-guts, while at pH 9 in the posterior mid-gut. The highest pH activity of carboxypeptidase B was 9 in 3 portions of midgut.The total carbohydrates of anterior mid-gut in the adult stage of Trachyderma philistine is 39.2 mg/ml, middle mid-gut is 34.04 mg/ml and posterior mid-gut is 34.9mg/ml .The highest activity of α- glucosidase was at pH 3.6 in anterior and posterior mid-guts while at 4.6 in middle midgut. The highest activity of β glucosidase was at pH 3.6 in anterior and posterior mid-guts while at 4.6 in middle mid-gut. The highest activity of α-galactosidase was at pH 3.6 in 3 portions of midguts. The highest activity of α – amylase is at pH 4.6 in both anterior mid-gut and middle mid-guts, while at pH 3.6 in posterior mid-gut.

Keywords: Amylase, Carbohydrases, Coleoptera, Midgut, Proteases, Trachyder-maphilistine
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