Sisal farming is a vital large-scale cash crop activity in Rongai Sub-County, Kenya, with over 1,000 hectares of land dedicated to its cultivation. As a primary economic activity, it provides income and employment opportunities for the local population. However, sisal processing industries are significant consumers of water, which has led to their establishment near natural water sources, including streams and rivers. This study was conducted to assess the impact of sisal processing industries on water resources in Rongai Sub-County, Kenya. The research focused on determining the effects of waste on water quality in the Molo River, Rongai River, MajaniMingi River, and surrounding boreholes. Data collection involved collecting water samples from the three rivers and adjacent boreholes. Laboratory analyses were performed to evaluate water quality parameters such as pH, dissolved oxygen (DO), temperature, conductivity, and total dissolved solids (TDS). Quantitative data from the laboratory results were compared with World Health Organization (WHO) and Kenyan water quality standards. The findings revealed that sisal processing activities impact local water resources. Water quality tests indicated notable alterations in pH, reduced DO levels, and slightly elevated TDS and conductivity in water bodies adjacent to processing facilities. There were no notable effects on water turbidity. These changes suggest increased pollution, potentially affecting aquatic life and human health. This study recommends a sustainable approach that involves integrating advanced waste treatment, community education, and innovative waste repurposing strategies which can reduce water pollution and health risks while enhancing environmental sustainability and regional economic resilience.