The present study aimed at understanding the connection between surface water, groundwater and heavy metal contamination. To achieve our goal, thirty samples of water collected from different water stations were analyzed for heavy metals at the toxicological laboratory of the national agricultural institute of Benin (INRAB). The results revealed a contamination of water samples with copper, zinc and nickel with concentrations lower than the safety limits by WHO which are respectively 3 mg/L, 5 mg/L and 0.05 mg/L. However, lead mean concentrations in stored and drilling water were above permissive value (0.04). In respect of cadmium, higher values were recorded in some drillings and surface waters. This may be due to the infiltration of heavy metals usually concentrated largely in sediments into drilling and groundwater. To face the challenge of public health problem posed by the contamination with heavy metals, it is important to implement new agricultural and sanitation policies