The purpose of this work was to examine the effectiveness of essential oils for inhibition of growth of micro organisms by the paper disc agar diffusion method. Essential oils from (Clococynth, Lupin, Castor, Snak) were evaluated on gram positive bacteria (staphylococcus aureus) and gram negative bacteria (pseudomonas aeruginosa) and compared with standard antibiotic ampiciline. From the results, the four essential oils showed the greatest inhibition (inhibition zone from 31 to 14) mm to staphylococcus aureus and (inhibition zone from 22 to 11) mm to pseudomonas aeruginosa in minimum inhibitory concentration (MIC) ranged (50-100)µLml.