Three varieties Sapindus trifoliatus Linn seed of Bangladesh were collected from local market like Gaibandha, Rajshahi and Dinajpur for comparative study. The proximate analysis of the collected seeds, physico-chemical characteristics and compositional analysis of the essential oils as well as the fatty oils were investigated. Essential oils were extracted by hydro distillation and fatty oils by solvent extraction, using 40-60 0C petroleum ether as solvent. On the dry sample basis Sapindus found to be containing essential oil is 0.025% in seeds of Gaibandha and Rajshahi, other sample contain 0.024% of the same. The fatty oil content was found to be 8.925%, 8.655%, 8.717% w/w in the seeds of Gaibandha, Rajshahi and Dinajpur respectively. Physical and chemical characteristics of the oils were determined by the usual standard procedures. The chemical composition of the essential oils and fatty oils were determined by Fourier Transform Infrared (FTIR) spectroscopy and Gas Chromatography-Mass Spectroscopy (GC-MS). The elemental nutrients in the seed cakes were determined by Energy Dispersive-X-ray Fluorescence (ED-XRF) instrument.