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March 2019 Vol. 7 No.3

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Abasi L
Alsraiheen K

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Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 7(3) pp. 119-122, March, 2019 

Copyright © 2019 Merit Research Journals
DOI: 10.5281/zenodo.2604700

Original Research Article

Immunological profile (GAD, ICA, insulin receptor antibodies) in type Ι DM patients, our experience at KHMC


Dr. Lamees Abasi*, Dr. Bahieh Al-abadi, Dr. Mansour Al-hiary, Dr. Maha Al-amer and Dr. Khaldoun Alsraiheen


MD. Specialist Clinical Pathology/immunology, King Hussein Medical Center, Amman-Jordan

*Corresponding Author’s E-mail: lameesabasi@yahoo.com
Mob: 00962772572233

Accepted March 05, 2019




The aim of this study was to determine the frequency of autoantibodies among Jordanian patients with diabetes mellitus type 1. A total of 128 subjects were included with age range between 1year and 14 years, 45.3% (58) of which were female and 54.7% (70) were male. Samples were analysed for anti-Glutamic acid decarboxylase, anti-insulin, and anti-islet cell antibodies using mosaic pancreas (monkey)/cerebellum (monkey) instructions for the indirect immunofluorescence assay, Euroimmun, Germany. One hundred and twenty eight subjects with T1DM were enrolled in study; 58 female (45.3 %) and 70 male (54.7%). The overall frequency of any autoantibodies was 36.7% (47 of 128 subjects).Anti-GAD, anti- Islet cell and anti-insulin antibodies were detected in 30.46% (39 subjects), 25.78% (33 subjects), and 23.43% (30 subjects) respectively table-1. Anti-GAD and anti-islet cell antibodies were more frequent in male, and anti-insulin antibodies were more frequent in female's figure 1, 2, 3. Frequency of multiple antibodies more than one was observed in 38 patients (29.68%); whereas only one antibody (anti-insulin antibodies) was observed in 28 patients (21.87%). 18 of 19 subjects with positive anti-GAD antibodies had positive anti-islet cell antibodies. About one third of diabetes mellitus patients from Jordan had presence of autoantibodies such as anti-GAD, anti-islet cell, and anti-insulin in a single form or in a combination, which is suggest of having diabetes mellitus type 1. We recommend further large studies to be done in different areas of country.

Keywords: Diabetes mellitus, Autoimmunity, Autoantibodies







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