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March 2018 Vol. 6 No.3

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Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 6(3) pp. 089-099, March, 2018 

Copyright © 2018 Merit Research Journals


Original Research Article

Study of the Biological Effects of Aqueous Extracts of Schinus terebinthifolius Raddi, on the survival fraction of mutant and wild strains of Escherichia coli

 
 
 

1Silva E. J., 1Nascimento C. C. H. C., 1,2Nascimento S. F., 1,4Vasconcelos, S. D. D. De, 5Nogueira R. I., 3Stephens P. R. S., 1Barreto A. S. and *1,2Diré G. F.

 

1LaboratoryofChemicalandBiologicalAnalysis (LAQB), Foundation StateUniversity Center ofthe West Zone (UEZO), Avenue Manuel Caldeira de Alvarenga, Campo Grande, Rio de Janeiro, Brazil.
2Estácio de Sá University (UNESA), Rio de Janeiro, Brazil.
3Laboratory of Innovations in Therapies, Teaching and Bioproducts/LITEB, OswaldoCruz Institute, IOC/ FIOCRUZ), Rio de Janeiro, Brazil.
4Federal Center ofTechnologicalEducation Celso Suckow da Fonseca- CEFET-NI, Rio de Janeiro, RJ- Brazil
5Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Rio de Janeiro, Brazil.

*Corresponding Author’s E-mail: Email:glauciodire@hotmail.com

Accepted March 03, 2018

 

Abstract

 

The plants have been used by man for millennia without effective study that proves the exposure of the population to chemical substances and toxic agents present in these phytotherapeutic compounds. In this respect, biological tests allow the evaluation of these substances and their efficiency, in many cases, generally attributed to the various parts of the species used, as well as generating mechanisms that support and / or protect the population from exposure to the active principles that constitute such specimens. Schinus terebinthifolius Raddi belongs to the family Anacardiaceae, popularly known as "Aroeira", it is one of the 71 species of plants related in Renisus, being attributed to this species antimicrobial, anti-inflammatory, antifungal and insecticide actions. These activities are related to their chemical constitutions by having secondary metabolites, most of them represented by flavonoids, such as tannins, essential oils, saponins, alkaloids, terpenes and sesquiterpenes, substances which present in various parts of the plant, such as branches and leaves. As the majority of these substances are related to several popular uses. The objective of the present study was to evaluate the biological effects of aqueous extracts of Schinus terebinthifolius Raddi, on the survival fractions of mutant and wild strains of Escherichia coli. Branches and leaves were collected, selected, processed and extracted by infusion to obtain the extracts, the extracts were used in two strains of E. coli by the disc diffusion method. A 0.9% NaCl solution was used as the negative control. For antibacterial evaluation the positive control was attributed to antibiotics present in RENAME and in the antioxidant activity to stannous chloride and hydrogen peroxide. From the obtained results it can be concluded that the aqueous extracts contain phytochemical constituents that potentiated the effect of the antibiotics. In relation to the types of extracts studied, the dehydration presented the formation of inhibition halos for the mutant strain BW 9091, unlike the treatments with the in natura that there was no formation of inhibition halos, for both strains; which is related to the differences in concentrations of both extracts.

Keywords: Schinus terebinthifolius Raddi, Escherichia coli, Aqueous Extract, Phytotherapy, Rename, Renisus.














































 










 







































 










 

 
 
   
   
   
   
   
   
   
   
   
   
   
 
 
 
 
 
 
 
 
   
 
                         

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