Numan KARAARSLAN1*, Ibrahim YILMAZ2,
Feride Sinem AKGUN3, Tezcan CALISKAN1,
Abdullah Talha SIMSEK4, Necati KAPLAN5,
Yasin EMRE KAYA6, Duygu YASAR SIRIN7,
Hanefi OZBEK8 and Ozkan ATES9
Prof. M.D., Namik Kemal University School of Medicine,
Department of Neurosurgery, 59100, Tekirdag, Turkey.
2Medical Pharmacologist, Pharmacist, Istanbul Medipol
University School of Medicine, Department of Medical
Pharmacology, 34810, Istanbul, Turkey.
3Assist. Prof. M.D., Istanbul Maltepe University
School of Medicine, Department of Emergency Medicine, 34857,
4Sepecialist M.D., Republic of Turkey, Ministry of
Health, State Hospital, Department of Neurosurgery, 36200, Kars,
5Assist. Prof. M.D.; Istanbul Rumeli University,
Corlu Reyap Hospital, Department of Neurosurgery, 59680,
6Assist. Prof. M.D., Abant Izzet Baysal Univesity
School of Medicine, Department of Orthopaedic and Traumatology,
13100, Bolu, Turkey.
7Assist. Prof. Ph.D., Namik Kemal University Faculty
of Arts and Sciences, Department of Molecular Biology and
Genetics, 59100, Tekirdag, Turkey.
8Prof. M.D., Ph.D., Istanbul Medipol University
School of Medicine, Department of Medical Pharmacology, 34810,
9Prof. M.D., Istanbul Esenyurt University, Esencan
Hospital, Department of Neurosurgery, 34510, Istanbul, Turkey.
*Corresponding Author’s É-mail: email@example.com
Phone: 282- 2507140
Fax number: 282-2509950
Accepted March 19, 2018
disc tissue is damaged and loses its normal physiological
functions due to age-related degeneration, vigorous physical
activity and trauma in all age groups and at any stage of life.
Here we aim to present how to prepare human primary cell
cultures effectively from intact intervertebral disc tissues
obtained from the patients undergoing operations for spinal
trauma and, from degenerated disc tissues obtained from patients
with a lumbar herniated. In addition, it is aimed to explain
bio/pharmaco-molecular methods used in their analyses at a
molecular level in line with the current literature. Articles
included in Pubmed between June 1988 and January 31, 2018, were
searched with the key words “nucleus pulposus cell
proliferation” and/or “annulus fibrosus” without language
restriction. Data of descriptive statistics were analyzed using
mean ± standard deviation or frequency (%) and demonstrated
using Microsoft Office 2010 and Excel. In addition, it was
explained how to obtain human primary cell culture from the
tissues taken from two different localizations of the single
intervertebral disc. A total of 419 articles were found to be
potentially relevant, as a result of first search. In this
literature review, cell cultures, which could possibly be the
main focus of cell therapies in the field of neurosurgery in the
near future, as well as current data were discussed from a
molecular point of view.
Keywords: Annulus fibrosus, Acridine orange, Propidium
iodide staining, differentiation, nucleus pulposus, primary cell
culture technique, proliferation.