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October 2017 Vol. 5 No.10

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Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 5(10) pp. 477-487, October, 2017 

Copyright © 2017 Merit Research Journals

Original Research Article

How Scaffolds, which are Polymeric Drug Delivery Systems Allowing Controlled Release, can be Tested in Human Primary Nucleus Pulposus and Annulus Fibrosus Cell Cultures?


Yener Akyuva1, Numan Karaarslan2*, Ibrahim Yılmaz3, Hanefi Ozbek4, Duygu Yasar Sırın5, Mehmet Sabri Gurbuz6, Yasin Emre Kaya7, Necati Kaplan8 and Ozkan Ates9


1M.D. Gaziosmanpasa Taksim Training and Research Hospital, Department of Neurosurgery, 34433, Istanbul, Turkey
2Assist. Prof. M.D.; Namik Kemal University School of Medicine, Department of Neurosurgery, 59100, Tekirdag, Turkey
3Medical Pharmacologist, Pharmacist; Istanbul Medipol University, School of Medicine, Department of Medical Pharmacology, 34810, Istanbul, Turkey
4Prof. M.D., Ph.D.; Istanbul Medipol University, School of Medicine, Department of Medical Pharmacology, 34810, Istanbul, Turkey
5Assist. Prof. Ph.D.; Namik Kemal University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, 59100, Tekirdag, Turkey
6Assist. Prof. M.D., Istanbul Medeniyet University School of Medicine, Department of Neurosurgery, 34000, Istanbul, Turkey
7Assist. Prof. M.D., Abant Izzet Baysal University School of Medicine, Department of Orthoaedic and Traumatology, 14000, Bolu, Turkey
8Assist. Prof. M.D., Istanbul Rumeli University, Corlu Reyap Hospital, Department of Neurosurgery, 59860, Tekirdag, Turkey
9Prof. M.D., EsenyurtUniversity, EsencanHospital, Department of Neurosurgery, 34517, Istanbul, Turkey

*Corresponding Author’s Émail: numikara@yahoo.com
Pbx: +9028 2250 5500

Accepted September 28, 2017




Promising pharmacological treatment of disk degeneration depends on development of appropriate materials and obtaining viable and proliferative nucleus pulposus/annulus fibrosus tissues with preservation of extracellular matrix compounds. This study aims to explain how drug and/or similar material impregnated polymeric scaffolds can be tested using primary cell cultures derived from human nucleus pulposus and annulus fibrosus tissues at pharmacomolecular level. For this purpose; we first explained how to prepare human primary cell culture setups. We remarked the physicochemical parameters for testing drugs and scaffolds containing drug-like substances. How to conduct the molecular analyzes of samples of cell cultures was explained. In this study; we explained very basically how to make in vitro analyzes for the development of the ideal drugs and/or polymeric biomaterials that can be used in clinical practice for the purpose of preventing disc degeneration. Moreover, medical pharmacologists, orthopedic and brain surgeons taking part in such studies can set up and analyze their own studies.

Keywords: Annulus fibrosus/ nucleus pulposus culture, controlled release, polymeric scaffolds






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