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April 2018 Vol.6 No.3

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El-Debaiky SA
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Merit Research Journal of Microbiology and Biological Sciences (ISSN: 2408-7076) Vol. 6(3) pp. 022-031, April, 2018

Copyright © 2018 Merit Research Journals

Original Research Article

Detection of T-2 Toxin from Some Egyptian Fusarium Strains with Special Reference to its Cytotoxicity

 
 
 

Samah Abd El-Kader El-Debaiky1*, Osman El-Maghraby2, Samia El-Rabbat3 and Ashraf Haider4

 

1Lecturer of Mycology, Botany Department, Faculty of Science, Tanta University
2Professor of Microbiology, Botany and Microbiology Department, Faculty of Science, Sohag University
3Researcher in Center of Scientific Excellence and Technology
4Professor of Molecular Genetics, Botany Department, Faculty of Science, Tanta University.

*Corresponding Author’s E-mail: Samaheldebaiky@science.tanta.edu.eg

Accepted April 02, 2018

 

Abstract

 

In the present study some Egyptian Fusarium strains were isolated previously from some cereal grains, tested for production of trichothecenes mycotoxins especially T-2 toxin by thin layer chromatography method (TLC). The results revealed that nine strains gave positive results for production of trichothecenes especially T-2 toxin. These strains are F. anthophylum, F. equiseti (KM246255.1), F. fujikuroi (MG211161), F. fujikuroi (MG211162), F. sporotrichioides (MG211163), F. solani (MG211159), F. solani (MG211160), F. subglutinans and F. verticillioides (XM_018895258.1). The identity percentage of T-2 toxin produced by each strain was detected by high performance thin layer chromatography (HPTLC) where F. solani (MG211159), F. fujikuroi (MG211161) and F. verticillioides (XM_018895258.1) recorded the highest values (95, 98 and 98 %) respectively. Moreover, F. solani (MG211159) was selected for mass production of T-2 toxin on rice grains then separated and purified using column chromatography. Then, cytotoxicity of this purified T-2 toxin was examined on fibroblast cell line to detect the safe concentration of this mycotoxin when consumed by human in contaminated cereal grains. The disorder changes and toxicity of treated cells reduced by decreasing T-2 toxin concentration till certain concentration where the appearance of the treated cells resembles the normal cells. This concentration which equal 5.60 ng/ml was considered safe and nontoxic. All these results revealed that the contamination of cereal grains by the tested Fusarium strains will be consequently contaminated by T-2 toxin which has toxic effects on human health except at the safe concentration.

Keywords: Fusarium, Trichothecenes, T-2 toxin, TLC, HPTLC and Cell lines.
























 
 









 
 

 
 
   
   
   
   
   
   
   
   
   
   
   
 
 
 
 
 
 
 
 
   
 
                         

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