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January 2018 Vol. 6 No.1

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Sharaby A
AL-Dhafer Z  

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Merit Research Journal of Agricultural Science and Soil Science (ISSN: 2350-2274) Vol. 6(1) pp. 007-012, January, 2018

Copyright © 2018 Merit Research Journals

Original Research Article

Successful Laboratory Mass Production of the American Bollworm Helicoverpa (armegira Hubn.) on Artificial Diet


Aziza Sharaby*1 and Zamzam AL-Dhafer2


1National Research Center Cairo, Egypt, Pests and Plant Protection Department
2Department of Biology College of Science Imam Abdulrahman Bin Faisal University P. O. Box Dammam Saudi Arabia

*Corresponding Author’s E-mail: Sharaby_aziza@yahoo.com

Accepted January 12, 201




The ever increasing demand for a large numbers of laboratory reared insects has necessitated the development of efficient and economic methods of production. The American bollworm or Tomato fruitworm Helicoverpa armigera (Hubn.) (Lepidoptera: Noctuidae) has long been recorded as a pest of many crop in many countries of the world, like Tomato, Maize, Zucchini, Green paper, Gourd, Muskmelon, Okra, Cassia, Potato, Bean, Chickpea, Sunflower, Red beet, Tobacco, Cowpea, Turnip. It also attacks the cultivated cotton plants. H. armigera can be successfully reared on artificial diet for the continuous maintenance of laboratory colonies and to facilitate investigations into different studies. The artificial diet composed of dry powdered chickpea, brewer's yeast, agar, ascorbic acid and mould inhibitor. The addition of vitamin B12 and riboflavin to the diet significantly increased the percentage of survivor larvae, moths fecundity, larval weight, fertility and moths fecundity, while the addition of sesame oil as a feeding stimulant significantly increased the pupal weight, percentage of deformed pupae and adults, these as a result of the fat tissues accumulation in the pupae during the feeding period of larvae . The pupae was passing through a period of diapauses from December to February, where the pupal period lasted about 100 day during this period for H. armigera of Saudi Arabia strain.

Keywords: Helicoverpa (Heliothes) armigera, successful artificial diet, rearing technique







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